human nucleus pulposus cells cat no: cp-h097  (Procell Inc)

Journal: Frontiers in Cell and Developmental Biology

Article Title: 17β-estradiol maintains extracellular matrix homeostasis of nucleus pulposus cells by activating p70 S6K1 signaling pathway

doi: 10.3389/fcell.2025.1564458

Figure Lengend Snippet: E2 inhibits MMPs and cleaved caspase 3 expression and reduces the ECM degradation. The rat IVD sections shown from left to right: sham ovariectomy (Sham), needle puncture plus ovariectomy with vehicle injection (OVX + Veh), and needle puncture plus ovariectomy with estradiol hormone replacement injection (OVX + E2) groups. (A, C, D) The expression of aggrecan and collagen II, which are the main components of ECM in NP tissues stained by immunohistochemical staining. (B, E, F) The expression of MMP 3 and cleaved caspase 3 in NP tissues stained by immunohistochemical staining. Values are mean ± SD (n = 5). ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. IVD, intervertebral disc; OVX, ovariectomy; veh, vehicle injection; E2, 17β-estradiol; MMP, matrix metalloproteinase; ECM, extracellular matrix; NP, nucleus pulposus; SD, standard deviation.

Article Snippet: We purchased human nucleus pulposus cells (Cat NO: CP-H097) from Procell Life Science& Technology (Wuhan, China) and cultured them in DMEM/F12 (Solarbio, China) containing 10% fetal bovine serum (Gibco, Australia) and penicillin (100 U/ml)-streptomycin (100 μg/mL) (Solarbio, China) and then placed in an incubator at 37°C with 5% CO 2 .

Techniques: Expressing, Injection, Staining, Immunohistochemical staining, Standard Deviation

Journal: Frontiers in Cell and Developmental Biology

Article Title: 17β-estradiol maintains extracellular matrix homeostasis of nucleus pulposus cells by activating p70 S6K1 signaling pathway

doi: 10.3389/fcell.2025.1564458

Figure Lengend Snippet: Immunohistochemistry showing that E2 mitigates IVDD by activating the p70 S6K1 signaling pathway downstream of mTOR in vivo . The rat IVD sections shown from left to right: sham ovariectomy (Sham), needle puncture plus ovariectomy with vehicle injection (OVX + Veh), and needle puncture plus ovariectomy with estradiol hormone replacement injection (OVX + E2) groups. (A) The levels of p70S6K1 and p-S6 in NP tissues of rat tails were detected using immunohistochemistry. (B, C) Data analysis of the immunohistochemistry. Values are mean ± SD (n = 5). ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001. IVD, intervertebral disc; IVDD, intervertebral disc degeneration; OVX, ovariectomy; veh, vehicle injection; E2, 17β-estradiol; NP, nucleus pulposus; p-S6, Phospho-S6 (the ribosomal protein S6); SD, standard deviation.

Article Snippet: We purchased human nucleus pulposus cells (Cat NO: CP-H097) from Procell Life Science& Technology (Wuhan, China) and cultured them in DMEM/F12 (Solarbio, China) containing 10% fetal bovine serum (Gibco, Australia) and penicillin (100 U/ml)-streptomycin (100 μg/mL) (Solarbio, China) and then placed in an incubator at 37°C with 5% CO 2 .

Techniques: Immunohistochemistry, In Vivo, Injection, Standard Deviation

Journal: Frontiers in Cell and Developmental Biology

Article Title: 17β-estradiol maintains extracellular matrix homeostasis of nucleus pulposus cells by activating p70 S6K1 signaling pathway

doi: 10.3389/fcell.2025.1564458

Figure Lengend Snippet: E2 activates the p70 S6K1 signaling pathway in vitro . (A–D) Western blotting was used to analyze the levels of p70 S6K1, p-S6K1 and p-S6 in human NPCs. (E, G) The level of p70 S6K1 in human NPCs by immunofluorescence staining. (F, H) The level of p-S6 in human NPCs by immunofluorescence staining. Values are mean ± SD (n = 3). ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. E2, 17β-estradiol; NPCs, nucleus pulposus cells; IL-1β, interleukin-1β; PF, PF4708671 (an p70 S6K1 inhibitor); p-S6, Phospho-S6 (the ribosomal protein S6); SD, standard deviation.

Article Snippet: We purchased human nucleus pulposus cells (Cat NO: CP-H097) from Procell Life Science& Technology (Wuhan, China) and cultured them in DMEM/F12 (Solarbio, China) containing 10% fetal bovine serum (Gibco, Australia) and penicillin (100 U/ml)-streptomycin (100 μg/mL) (Solarbio, China) and then placed in an incubator at 37°C with 5% CO 2 .

Techniques: In Vitro, Western Blot, Immunofluorescence, Staining, Standard Deviation

Journal: Frontiers in Cell and Developmental Biology

Article Title: 17β-estradiol maintains extracellular matrix homeostasis of nucleus pulposus cells by activating p70 S6K1 signaling pathway

doi: 10.3389/fcell.2025.1564458

Figure Lengend Snippet: E2 activation of p70 S6K1 signaling pathway improves ECM anabolism in vitro . (A–C) Western blot analysis was used to analyze the expression of Collagen II and Aggrecan in human NPCs. (D, F) The expression of Aggrecan in human NPCs by immunofluorescence staining. (E, G) The expression of Collagen II in human NPCs by immunofluorescence staining. Values are mean ± SD (n = 3). ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. E2, 17β-estradiol; NPCs, nucleus pulposus cells; IL-1β, interleukin-1β; PF, PF4708671(an p70 S6K1 inhibitor); ECM, extracellular matrix; SD, standard deviation.

Article Snippet: We purchased human nucleus pulposus cells (Cat NO: CP-H097) from Procell Life Science& Technology (Wuhan, China) and cultured them in DMEM/F12 (Solarbio, China) containing 10% fetal bovine serum (Gibco, Australia) and penicillin (100 U/ml)-streptomycin (100 μg/mL) (Solarbio, China) and then placed in an incubator at 37°C with 5% CO 2 .

Techniques: Activation Assay, In Vitro, Western Blot, Expressing, Immunofluorescence, Staining, Standard Deviation

Journal: Frontiers in Cell and Developmental Biology

Article Title: 17β-estradiol maintains extracellular matrix homeostasis of nucleus pulposus cells by activating p70 S6K1 signaling pathway

doi: 10.3389/fcell.2025.1564458

Figure Lengend Snippet: E2 activation of the p70 S6K1 signaling pathway inhibits MMP3 and cleaved caspase 3 in vitro . (A–D) Western blot analysis was used to analyze the expression of MMP3 and cleaved caspase 3. (E, F) The expression of MMP3 was determined by immunofluorescence staining. (G, H) The expression of cleaved caspase 3 was determined by immunofluorescence staining. Values are mean ± SD (n = 3). ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001. E2, 17β-estradiol; NPCs, nucleus pulposus cells; IL-1β, interleukin-1β; PF, PF4708671(an p70 S6K1 inhibitor); MMP3, matrix metalloproteinase 3; SD, standard deviation.

Article Snippet: We purchased human nucleus pulposus cells (Cat NO: CP-H097) from Procell Life Science& Technology (Wuhan, China) and cultured them in DMEM/F12 (Solarbio, China) containing 10% fetal bovine serum (Gibco, Australia) and penicillin (100 U/ml)-streptomycin (100 μg/mL) (Solarbio, China) and then placed in an incubator at 37°C with 5% CO 2 .

Techniques: Activation Assay, In Vitro, Western Blot, Expressing, Immunofluorescence, Staining, Standard Deviation